Assessing the Biological Significance of Primary Structure
Consensus Patterns Using Sequence Databanks. I. Heat-shock and
Glucocorticoid Control Elements in Eukaryotic Promoters
J.M. Claverie, I. Sauvaget
Nucleic Acids Research
1(2): 95-104 (1985)
Abstract
We describe FORTRAN 77 software allowing for convenient
searching of any segmented and ambiguous pattern in the
currently available protein or nucleotide sequence databanks. For
proteins, this software can be instrumental in defining conserved
functional domains among non-homologous overall primary
structures. For nucleic acids, it is used in detecting complex
and/or low consensus structural or regulatory patterns. As first
applications we have studied the distribution of short consensus
sequences believed to characterize heat-shock and glucocorticoid
regulated promoters. This analysis allowed an evaluation of the
specificity, probable role and thus biological significance of
various regions of these consensus. In addition, the expression of
several known genes are predicted to be heat-shock or
glucocorticoid sensitive.
